About Horizontal Electrophoresis Apparatus
It can be accommodate 6 paper strips horizontally .Buffer can be saturated very fast in this mechanism. A recessed safety interlock switch breaks the current of both electrodes while the lid of mechanism is uplifted. Both electrode section have capacity of 550ml and each are provided with transverse baffles to minimize. Diffusion of buffer which is in contact with the ends of paper strips. Polarity of the electrode is indicate by two pilot lamps provided on the front panel. The complete mechanism is fitted with leveling screws of facilitate leveling .The elementary unit consists of anode and cathode reservoir with platinum electrodes, special glass lid and power cord.
Precision and Durability Built InEach apparatus features pure platinum electrodes, ensuring stable current flow and exceptional corrosion resistance. The chamber is crafted from clear, impact-resistant acrylic and polycarbonate, providing leak-proof durability and allowing full observation of the run. This attention to material quality guarantees reliable, repeatable results in demanding laboratory conditions.
Versatile Gel Casting and Sample LoadingWith interchangeable gel trays (small, medium, and large) and combs (available in multiple thicknesses and well formats), users can efficiently adjust capacity and well number based on experimental demands. Easy-to-use casting dams and sample loading guides help streamline setup and ensure uniform lane formation, accommodating up to 20 samples per run.
Safety and Compatibility AssuredA safety interlock lid protects users by preventing operation if the chamber is open. The UV transparent gel trays simplify observation and imaging, while compatibility with standard electrophoresis power supplies allows integration into existing lab setups. The apparatus is designed for manual control, offering ambient to 60C operation (with optional cooling) and straightforward benchtop use.
FAQ's of Horizontal Electrophoresis Apparatus:
Q: How does the horizontal electrophoresis apparatus ensure user safety during operation?
A: Safety is assured through a safety interlock lid, which prevents the apparatus from operating when open, and impact-resistant polycarbonate components, protecting both the user and samples during routine laboratory procedures.
Q: What types of samples can be separated using this apparatus?
A: This horizontal electrophoresis system effectively separates DNA, RNA, and proteins, making it suitable for a range of molecular biology applications, including research, teaching, diagnostics, and nucleic acid analysis.
Q: When is it necessary to use the optional cooling feature?
A: The optional cooling feature should be employed when running prolonged electrophoresis experiments or high-voltage protocols where heat buildup may impact sample integrity or band resolution, especially for temperature-sensitive samples.
Q: Where is this electrophoresis system best utilized?
A: It is ideal for benchtop laboratory environments-whether in academic, research, clinical, or industrial labs-where reliable, high-throughput molecular separation is required.
Q: What is the process for setting up and loading samples into the gel?
A: Setup involves casting the gel in UV transparent trays using supplied dams, inserting the desired comb (1 mm or 1.5 mm), and filling sample wells. After assembling the chamber and connecting compatible power supplies, samples are carefully loaded with guides for precise application.
Q: How does the apparatus benefit molecular biology workflows?
A: The apparatus offers high-resolution separations, uniform field distribution, flexible sample capacity, and direct UV observation. Its manual and modular design simplifies workflow and adapts to diverse experimental needs without complex automation.
Q: What accessories and core components are included with the apparatus?
A: Every unit is supplied with gel casting dams, sample loading guides, various combs and gel trays, a user manual, and a leak-proof electrophoresis chamber fitted with corrosion-resistant platinum electrodes and a safety lid for comprehensive functionality.